Methodology

  • Cell culture, bacterial and yeast culture
  • Culture and study of opportunistic pathogens (filamentous fungi and bacteria)
  • Extraction, purification and analysis of lipids and sterols
  • Purification and analysis of bacterial lipopolysaccharide
  • Construction of vectors for expression of genes in various hosts (bacteria, yeast)
  • Confocal and epifluorescence microscopy
  • Protemics analysis
  • Productrion of recombinant proteins
  • Production and purification of RNA natively and in vitro
  • Purification of proteins at analytical scale (immunoprecipitation, TAP) and preparative scale using Akta Pure system
  • Electrophoresis and analysis of proteins (staning, activity, Western blot)
  • Yeast genetics and phenotypic assays
  • Mitochondrial activity assessement (ROS production, oxymetry, proteomics)
  • Molecular biology (PCR, cloning, directed mutagenesis)
  • Enzymatic studies