Complete list of publications

2021-

Grob, G., Hemmerle, M., Yakobov, N., Mahmoudi, N., Fischer, F., Senger, B., and Becker, H.D. (2022). tRNA-dependent addition of amino acids to cell wall and membrane components. Biochimie. https://doi.org/10.1016/j.biochi.2022.09.017
Yakobov, N., Mahmoudi, N., Grob, G., Yokokawa, D., Saga, Y., Kushiro, T., Worrell, D., Roy, H., Schaller, H., Senger, B., et al. (2022). RNA-dependent synthesis of ergosteryl-3β-O-glycine in Ascomycota expands the diversity of steryl-amino acids. J Biol Chem 298, 101657.
Verdura, E., Senger, B., Raspall-Chaure, M., Schlüter, A., Launay, N., Ruiz, M., Casasnovas, C., Rodriguez-Palmero, A., Macaya, A., Becker, H.D., et al. (2022). Loss of seryl-tRNA synthetase (SARS1) causes complex spastic paraplegia and cellular senescence. J Med Genet, jmedgenet-2022-108529.
Hemmerle, M., Senger, B., Kucharczyk, R., and Becker, H.D. (2022). Visualizing Mitochondrial Importability of a Protein Using the Yeast Bi-Genomic Mitochondrial-Split-GFP Strain and an Ordinary Fluorescence Microscope. Methods Mol Biol 2497, 255-267.
Yokokawa, D., Tatematsu, S., Takagi, R., Saga, Y., Roy, H., Fischer, F., Becker, H. D., & Kushiro, T. (2021). Synthesis of aminoacylated ergosterols: A new lipid component of fungi. Steroids, 169(February), 11–14. 10.1016/j.steroids.2021.108823
Enkler, L., Rinaldi, B., De Craene, J. O., Hammann, P., Nureki, O., Senger, B., Friant, S., & Becker, H. D. (2021). Cex1 is a component of the COPI intracellular trafficking machinery. Biology Open, 10(3), 1–10. 10.1242/BIO.058528

2017-2020

Yakobov, N., Fischer, F., Mahmoudi, N., Saga, Y., & Grube, C. D. (2020). RNA-dependent sterol aspartylation in fungi. Proceedings of the National Academy of Sciences. 10.1073/pnas.2003266117
Bader, G., Enkler, L., Araiso, Y., Hemmerle, M., Binko, K., Baranowska, E., De Craene, J. O., Ruer-Laventie, J., Pieters, J., Tribouillard-Tanvier, D., Senger, B., Rago, J. P. Di, Friant, S., Kucharczyk, R., & Becker, H. D. (2020). Assigning mitochondrial localization of dual localized proteins using a yeast bi-genomic mitochondrial-split-gfp. ELife, 9, 1–24. 10.7554/eLife.56649
Manole, A., Efthymiou, S., O’Connor, E., Mendes, M. I., Jennings, M., Maroofian, R., Davagnanam, I., Mankad, K., Lopez, M. R., Salpietro, V., Harripaul, R., Badalato, L., Walia, J., Francklyn, C. S., Athanasiou-Fragkouli, A., Sullivan, R., Desai, S., Baranano, K., Zafar, F., … Houlden, H. (2020). De Novo and Bi-allelic Pathogenic Variants in NARS1 Cause Neurodevelopmental Delay Due to Toxic Gain-of-Function and Partial Loss-of-Function Effects. American Journal of Human Genetics, 107(2), 311–324. 10.1016/j.ajhg.2020.06.016
Hemmerle, M., Wendenbaum, M., Grob, G., Yakobov, N., Mahmoudi, N., Senger, B., Debard, S., Fischer, F., & Becker, H. D. (2020). Noncanonical inputs and outputs of tRNA aminoacylation. In The enzymes : Biology of Aminoacyl-tRNA Synthetases (Issue 48, pp. 1–423).
Yakobov, N., Debard, S., Fischer, F., Senger, B., & Becker, H. D. (2018). Cytosolic aminoacyl-tRNA synthetases: Unanticipated relocations for unexpected functions. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1861(4), 387–400. https://doi.org/10.1016/j.bbagrm.2017.11.004
Debard, S., Bader, G., De Craene, J. O., Enkler, L., Bär, S., Laporte, D., Hammann, P., Myslinski, E., Senger, B., Friant, S., & Becker, H. D. (2017). Nonconventional localizations of cytosolic aminoacyl-tRNA synthetases in yeast and human cells. Methods, 113, 91–104. doi.org/10.1016/j.ymeth.2016.09.017

2015 à 2016

Chaliotis A, Vlastaridis P, Mossialos D, Ibba M, Becker HD, Stathopoulos C, Amoutzias GD. (2016) The complex evolutionary history of aminoacyl-tRNA synthetases, Nucleic Acids Res.; pii: gkw1182.
Debard S, Bader G, De Craene JO, Enkler L, Bär S, Laporte D, Hammann P, Myslinski E, Senger B, Friant S, Becker HD. (2016) Nonconventional localizations of cytosolic aminoacyl-tRNA synthetases in yeast and human cells, Methods. pii: S1046-2023(16)30335-8. doi: 10.1016/j.ymeth.2016.09.017.
Ibrahim M, Gahoual R, Enkler L, Becker HD, Chicher J, Hammann P, François YN, Kuhn L, Leize-Wagner E. Improvement of Mitochondria Extract from Saccharomyces cerevisiae Characterization in Shotgun Proteomics Using Sheathless Capillary Electrophoresis Coupled to Tandem Mass Spectrometry. J Chromatogr Sci. 2016;54(4):653-63. doi: 10.1093/chromsci/bmw005.
Apostolidi M, Saad NY, Drainas D, Pournaras S, Becker HD, Stathopoulos C. A glyS T-box riboswitch with species-specific structural features responding to both proteinogenic and nonproteinogenic tRNAGly isoacceptors. RNA. 2015;21(10):1790-806. doi: 10.1261/rna.052712.115.

2013 à 2014

Frechin M, Enkler L, Tetaud E, Laporte D, Senger B, Blancard C, Hammann P, Bader G, Clauder-Münster S, Steinmetz LM, Martin RP, di Rago JP, Becker HD. Expression of nuclear and mitochondrial genes encoding ATP synthase is synchronized by disassembly of a multisynthetase complex. Mol Cell. 2014; 56(6):763-76. doi: 10.1016/j.molcel.2014.10.015.
Laporte D, Huot JL, Bader G, Enkler L, Senger B, Becker HD. Exploring the evolutionary diversity and assembly modes of multi-aminoacyl-tRNA synthetase complexes: lessons from unicellular organisms. FEBS Lett. 2014;588(23):4268-78. doi: 10.1016/j.febslet.2014.10.007.
Araiso Y, Huot JL, Sekiguchi T, Frechin M, Fischer F, Enkler L, Senger B, Ishitani R, Becker HD, Nureki O. Crystal structure of Saccharomyces cerevisiae mitochondrial GatFAB reveals a novel subunit assembly in tRNA-dependent amidotransferases. Nucleic Acids Res. 2014;42(9):6052-63. doi: 10.1093/nar/gku234. Epub 2014 Apr 1.
Huot JL, Enkler L, Megel C, Karim L, Laporte D, Becker HD, Duchêne AM, Sissler M, Maréchal-Drouard L. Idiosyncrasies in decoding mitochondrial genomes. Biochimie. 2014 May;100:95-106. doi: 10.1016/j.biochi.2014.01.004. Epub 2014 Jan 16.
Saad NY, Stamatopoulou V, Brayé M, Drainas D, Stathopoulos C, Becker HD. Two-codon T-box riboswitch binding two tRNAs. Proc Natl Acad Sci U S A. 2013 Jul 30;110(31):12756-61. doi: 10.1073/pnas.1304307110. Epub 2013 Jul 15.
Nozawa K, Ishitani R, Yoshihisa T, Sato M, Arisaka F, Kanamaru S, Dohmae N, Mangroo D, Senger B, Becker HD, Nureki O. Crystal structure of Cex1p reveals the mechanism of tRNA trafficking between nucleus and cytoplasm. Nucleic Acids Res. 2013 Apr 1;41(6):3901-14. doi: 10.1093/nar/gkt010. Epub 2013 Feb 8.
Saad NY, Schiel B, Brayé M, Heap JT, Minton NP, Dürre P, Becker HD. Riboswitch (T-box)-mediated control of tRNA-dependent amidation in Clostridium acetobutylicum rationalizes gene and pathway redundancy for asparagine and asparaginyl-trnaasn synthesis. J Biol Chem. 2012 Jun 8;287(24):20382-94. doi: 10.1074/jbc.M111.332304. Epub 2012 Apr 13.
Fischer F, Huot JL, Lorber B, Diss G, Hendrickson TL, Becker HD, Lapointe J, Kern D. The asparagine-transamidosome from Helicobacter pylori: a dual-kinetic mode in non-discriminating aspartyl-tRNA synthetase safeguards the genetic code. Nucleic Acids Res. 2012 Jun;40(11):4965-76. doi: 10.1093/nar/gks167. Epub 2012 Feb 22.

2008 à 2012

Blaise, M., Bailly, M., Frechin, M., Behrens, M. A., Fischer, F., Oliveira, C. LP., Becker, H. D., Pedersen, J. S., Thirup, S. & Kern, D. Crystal structure of a transfer-ribonucleoprotein particle that promotes asparagine formation. EMBO2010 Sep 15;29(18):3118-29. Epub 2010 Aug 17
Frechin, M., Kern, D., Martin, R. P., Becker, H. D. & Senger, B. (2010) Arc1p: anchoring, routing, coordinating. FEBS Letters584, 427-433.
Frechin, M., Senger, B. Brayé, M., Kern, D., Martin, R. & Becker, H. D. (2009) Yeast mitochondrial Gln-tRNA^Gln is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS. Genes Dev. 23, 1119-1130
Giannouli, S., Kyritsis, A., Malissovas, N., Becker, H. D. & Stathopoulos, C. (2009) On the role of an unusual tRNA(Gly) isoacceptor in Staphylococcus aureus. Biochimie91,344-351.
Blaise, M., Olieric, V., Sauter, C., Lorber, B., Roy, B., Karmakar, S., Banerjee, R., Becker, H. D. & Kern, D. (2008) Crystal structure of glutamyl-queuosine tRNA^Asp synthetase complexed with L-glutamate: structural elements mediating tRNA-independent activation of glutamate and glutamylation of tRNA^Asp anticodon. J. Mol. Biol.381, 1224-1237.
Pujol, C., Bailly, M., Kern, D. Maréchal-Drouard, L., Becker, H. D. & Duchêne A-M (2008) Plant tRNA-dependent amidotransferase ensures mitochondrial and chloroplastic Gln-tRNA^Gln synthesisProc. Natl. Acad. Sci. U. S. A. 105, 6481-6485.
Bailly, M., Blaise, M., Roy, H., Deniziak, Lorber, B., Birck, C., Becker, H. D. & Kern, D. (2008). tRNA-dependent asparagine formation in prokaryotes: Characterization, isolation and structural and functional analysis of a ribonucleoprotein particle generating Asn-tRNA^Asn.Methods 44,146-163.

2003 à 2007

Touzé, E., Lorber, B., Deniziak, M. A., Becker, H. D., Kern, D., Giegé, R. & Sauter, C. (2007). Disoerder can exist inside well-diffracting crystals. Crystal growth & design 7,2195-2197.
Bailly, M., Blaise, M., Lorber, B., Becker, H. D., & Kern, D. (2007). The transamidosome: a ribonucleoprotein particle dedicated to prokaryotic tRNA-dependent asparagines biosynthesis. Mol. Cell 28,228-239.
Roy, H., Becker, H. D., Mazauric, M-H. & Kern, D. (2007). Structural elements defining elongation factor Tu mediated suppression of codon ambiguity. Nucleic Acids Res. 35,3420-3430.
Deniziak, M. A., Sauter, C., Becker, H. D., Paulus, C. A., Giegé, R. & Kern, D. (2007). Deinococcus glutaminyl-tRNA synthetase is a chimer between proteins from an ancient and the modern pathways of aminoacyl-tRNA formation.Nucleic Acids Res. 35, 1421-1431.
Bailly, M., Giannouli, S., Blaise, M., Stathopoulos, C., Kern D. & Becker, H. D. (2006). A single tRNA base-pair mediates bacterial tRNA-dependent biosynthesis of asparagines. Nucleic Acids Res. 34,6083-6094.
Blaise, M., Becker, H. D., Lapointe, J., Cambillau, C., Giegé, R. & Kern, D. (2005). Glu-Q-tRNA^Asp synthetase coded by the yadB gene, a new paralog of aminoacyl-tRNA synthetase that glutamylates tRNA^Asp anticodon. Biochimie87,847-861.
Kern D., Roy H. & Becker, H. D. (2005). Asparaginyl-tRNA synthetase, in The Aminoacyl-tRNA synthetases, edited by Michael Ibba, Christopher Francklyn and Stephen Cusack. Landes Bioscience, 193-209
Deniziak, M. A., Sauter, C., Becker, H. D., Giegé, R. & Kern, D. (2004). Crystallization and preliminary X-ray characterization of the atypical glutaminyl-tRNA synthetase from Deinococcus radiodurans. Acta Crystallogr. D Biol. Crystallogr.60, 2361-2363.
Blaise, M., Becker, H. D., Keith, G., Cambillau, C., Lapointe, J., Giegé, R. & Kern, D. (2004). A minimalist glutamyl-tRNA synthetase dedicated to aminoacylation of the tRNA^Asp QUC anticodon. Nucleic Acids Res.32, 2768-2775.
Dubois, D. Y., Blaise, M., Becker, H. D., Campanacci, V., Keith, G., Giegé, R., Cambillau, C., Lapointe, J. & Kern, D. (2004). An aminoacyl-tRNA synthetase-like protein encoded by the Escherichia coli yadB gene glutamylates specifically tRNA^Asp. Proc. Natl. Acad. Sci. U. S. A.101, 7530-7535.
Campanacci, V., Dubois, D. Y., Becker, H. D., Kern, D., Spinelli, S., Valencia, C., Pagot, F., Salomoni, A., Grisel, S., Vincentelli, R., Bignon, C., Lapointe, J., Giegé, R. & Cambillau, C. (2004).The Escherichia coli YadB gene product reveals a novel aminoacyl-tRNA synthetase like activity. J. Mol. Biol.337, 273-283.
Roy, H., Becker, H. D., Reinbolt, J. & Kern, D. (2003). When contemporary aminoacyl-tRNA synthetases invent their cognate amino acid metabolism. Proc. Natl. Acad. Sci. U. S. A.100, 9837-9842

1996 à 2002

Salazar, J-C., Zúñiga, R., Raczniak, G., Becker, H. D., Söll, D. & Orellana, O. (2001). A dual-specific Glu-tRNA^Gln^Asn amidotransferase is involved in decoding glutamine and asparagine codons in Acidithiobacillus ferrooxidans and Asp-tRNA. FEBS Letters500, 129-131.
Ruan, B., Ahel, I., Ambrogelly, A., Becker, H. D., Bunjun, S., Feng, L., Tumbula-Hansen, D., Ibba, M., Korencic, D., Kobayashi, H., Jacquin-Becker, C., Mejlhede, N., Min, B., Raczniak, G., Rinehart, J., Stathopoulos, C., Li, T. & Söll, D. (2001) Genomics and the evolution of aminoacyl-tRNA synthesis. Acta Biochim. Pol.48, 313-321.
Raczniak, G., Becker, H. D., Min, B. & Söll, D. (2001). A Single Amidotransferase Forms Asparaginyl-tRNA and Glutaminyl-tRNA in Chlamydia trachomatis. J. Biol. Chem.276, 45862-45867.
Stathopoulos, C., Ahel, I., Ali, K., Ambrogely, A., Becker, H. D., Bunjun, S., Feng, L., Herring, S., Jacquin-Becker, C., Kobayashi, H., Korencic, D., Krett, B., Mejlhede, N., Min, B., Nakano, H., Namgoong, S., Polycarpo, C., Raczniak, G., Rinehart, J., Rosas-Sandoval, G., Ruan, B., Sabina, J., Sauerwald, A., Toogood, H., Tumbula-Hansen, D., Ibba, M. & Söll, D. (2001). Aminoacyl-tRNA synthesis: a postgenomic perspective. Cold Spring Harb. Symp. Quant. Biol.66, 175-183.
Stathopoulos, C., Jacquin-Becker, C., Becker, H. D., Li, T,. Ambrogely, A., Longman, R. & Söll, D. (2001). Methanococcus jannaschii ProCysRS possesses overlapping amino acid binding sites. Biochemistry40, 46-52.
Söll, D., Becker, H. D., Plateau, P., Blanquet, S. & Ibba, M. (2000). Context-dependent anticodon recognition by class I lysyl-tRNA synthetases. Proc. Natl. Acad. Sci. U. S. A.97, 14224-14228.
Becker, H. D., Tumbula, D. L., Chang, W-Z. & Söll, D. (2000). Domain-specific recruitment of amide amino acids for protein synthesis. Nature407, 106-110.
Becker, H. D., Min, B., Jacobi, C., Raczniak, G., Pelaschier, J., Roy, H., Klein, S., Kern, D. & Söll, D. (2000). The heterotrimeric Thermus thermophilus Asp-tRNA^Asn amidotransferasecan also generate Gln-tRNA^Gln. FEBS Letters476, 140-144.
Ibba, M., Becker, H. D., Stathopoulos, C., Tumbula, D. L. & Söll, D. (2000). The adaptor hypothesis revisited.Trends Biochem. Sci.25, 311-316.
Becker, H. D., Roy, H., Moulinier, L., Mazauric, M-H., Keith, G. & Kern, D. (2000). Thermus thermophilus contains an eubacterial and an archaebacterial aspartyl-tRNA synthetase. Biochemistry39, 3216-3230
Stathopoulos, C., Li, T., Longman, R., Vothknecht, U. C., Becker, H. D., Ibba, M. & Söll, D. (2000). One polypeptide with two aminoacyl-tRNA synthetase activities. Science 287, 479-482
Tumbula, D. L., Vothknecht, U. C., Kim, H., Ibba, M., Min, B., Li, T., Pelaschier, J., Stathopoulos, C., Becker, H. D. & Söll, D. (1999). Archaeal Aminoacyl-tRNA Synthesis: Diversity Replaces Dogma. Genetics152, 1260-1276.
Becker, H. D. & Kern, D. (1998). Thermus thermophilus: a link in evolution of the tRNA-dependent amino acid amidation pathways. Proc. Natl. Acad. Sci. U.S.A.95, 12832-12837.
Becker, H. D., Reinbolt, J., Kreutzer, R., Giegé, R. & Kern, D. (1997). Existence of two distinct aspartyl-tRNA synthetases in Thermus thermophilus. Structural and biochemical properties of the two enzymes. Biochemistry36, 8785-8797.
Becker, H. D., Giegé R. & Kern D. (1996). Identity of Prokaryotic and Eukaryotic tRNA^Asp for Aminoacylation by Aspartyl-tRNA Synthetase from Thermus thermophilus.Biochemistry35, 7447-7458.