DyPS - Équipe Hubert Becker - GMGM - Génétique moléculaire, génomique, microbiologie - Université de Strasbourg https://gmgm.unistra.fr fr DyPS - Équipe Hubert Becker - GMGM - Génétique moléculaire, génomique, microbiologie - Université de Strasbourg Sun, 24 Nov 2024 14:20:31 +0100 Sun, 24 Nov 2024 14:20:31 +0100 TYPO3 EXT:news news-17028 Thu, 17 Oct 2024 19:40:13 +0200 New publication from the Becker & Tarassov-Smirnov teams: AARS Online: A collaborative database on the structure, function, and evolution of the aminoacyl-tRNA synthetases /en/news/piece-of-news/new-publication-from-the-becker-tarassov-smirnov-teams-aars-online-a-collaborative-database-on-the-structure-function-and-evolution-of-the-aminoacyl-trna-synthetases AARS Online is an interactive Wikipedia-like tool curated by an international consortium of aminoacyl-tRNA synthetase (aaRS) experts. Accessible at www.aars.online, it is a free resource to delve into the world of aaRSs, key players of the mRNA translation process. AARS Online systematizes existing knowledge about aaRSs by showcasing a taxonomically diverse selection of aaRS sequences and structures. Through its graphical user interface, it facilitates a seamless exploration between protein sequence and structure, providing a friendly introduction to the material for non-experts and a useful resource for experts. AARS Online: A collaborative database on the structure, function, and evolution of the aminoacyl-tRNA synthetases.

Jordan Douglas, Haissi Cui, John J. Perona, Oscar Vargas-Rodriguez, Henna Tyynismaa, Claudia Alvarez Carreño, Jiqiang Ling, Lluís Ribas de Pouplana, Xiang-Lei Yang, Michael Ibba, Hubert Becker, Frédéric Fischer, Marie Sissler, Charles W. Carter Jr, Peter R. Wills (2024) IUBMB Life, doi: 10.1002/iub.2911.

The aminoacyl-tRNA synthetases (aaRS) are a large group of enzymes that implement the genetic code in all known biological systems. They attach amino acids to their cognate tRNAs, moonlight in various translational and non-translational activities beyond aminoacylation, and are linked to many genetic disorders. The aaRS have a subtle ontology characterized by structural and functional idiosyncrasies that vary from organism to organism, and protein to protein. Across the tree of life, the 22 coded amino acids are handled by 16 evolutionary families of Class I aaRS and 21 families of Class II aaRS. We introduce AARS Online, an interactive Wikipedia-like tool curated by an international consortium of field experts. This platform systematizes existing knowledge about the aaRS by showcasing a taxonomically diverse selection of aaRS sequences and structures. Through its graphical user interface, AARS Online facilitates a seamless exploration between protein sequence and structure, providing a friendly introduction to the material for non-experts and a useful resource for experts. Curated multiple sequence alignments can be extracted for downstream analyses. Accessible at www.aars.online, AARS Online is a free resource to delve into the world of the aaRS.

]]> Actualités du GMGM Publications du GMGM Actualités de l'Équipe Becker Publications de l'Équipe Becker Actualités de l'Équipe Tarassov-Smirnov Publications de l'Équipe Tarassov-Smirnov news-16933 Tue, 24 Sep 2024 11:32:02 +0200 New publication : Monitoring mitochondrial localization of dual localized proteins using a Bi-Genomic Mitochondrial-Split-GFP /en/news/piece-of-news/new-publication-monitoring-mitochondrial-localization-of-dual-localized-proteins-using-a-bi-genomic-mitochondrial-split-gfp Autors : Solène Zuttion, Bruno Senger, Chiranjit Panja, Sylvie Friant, Róża Kucharczyk and Hubert Dominique Becker Photo : ©Jaime-Olivares

Abstract

Even if a myriad of approaches has been developed to identify the subcellular localization of a protein, the easiest and fastest way remains to fuse the protein to Green Fluorescent Protein (GFP) and visualize its location using fluorescence microscopy. However, this strategy is not well suited to visualize the organellar pools of proteins that are simultaneously localized both in the cytosol and in organelles because the GFP signal of a cytosolic pool of the protein (cytosolic echoform) will inevitably mask or overlay the GFP signal of the organellar pool of the protein (organellar echoform). To solve this issue, we engineered a dedicated yeast strain expressing a Bi-Genomic Mitochondrial-Split-GFP. This split-GFP is bi-genomic because the first ten ß-strands of GFP (GFPß1–10) are encoded by the mitochondrial genome and translated by mitoribosomes whereas the remaining ß-strand of GFP (GFPß11) is fused to the protein of interest encoded by the nucleus and expressed by cytosolic ribosomes. Consequently, if the GFPß11-tagged protein localizes into mitochondria, GFP will be reconstituted by self-assembly GFPß1–10 and GFPß11 thereby generating a GFP signal restricted to mitochondria and detectable by regular fluorescence microscopy. In addition, because mitochondrial translocases and import mechanisms are evolutionary well conserved, the BiG Mito-Split-GFP yeast strain can be used to probe mitochondrial importability of proteins regardless of their organismal origins and can thus serve to identify unsuspected mitochondrial echoforms readily from any organism.

]]> Actualités du GMGM Publications du GMGM Actualités de l'Équipe Becker Publications de l'Équipe Becker Actualités de l'Équipe Friant Publications de l'Équipe Friant news-16932 Tue, 24 Sep 2024 11:21:38 +0200 Spotlight RNA Society: Solène Zuttion /en/news/piece-of-news/spotlight-rna-society-solene-zuttion Article paru dans RNA Society sur Solène Zuttion, étudiante en thèse sous la co-direction d'Hubert Becker et de Sylvie Friant. Meet Solène Zuttion, a dedicated researcher currently pursuing a Ph.D. at the Integrative Molecular and Cellular Biology (IMCBio, Strasbourg, France) Graduate School of Research. Solène is passionate about the non-canonical functions of multi-localized aminoacyl-tRNA synthetases (aaRS) and is particularly fascinated by how these enzymes, primarily involved in cytosolic translation, can relocate to various organelles to perform diverse functions. Solène admires the research programs of Paul Fox (Cleveland Clinic, USA), Sunghoon Kim (Yonsei University, Korea), and Ignacio Luque (Spanish National Research Council, Spain), who focus on membrane-bound aaRSs. https://www.rnasociety.org/zuttion--solene Solène earned her bachelor's and master's degrees from the University of Strasbourg, where she was inspired by exceptional biochemistry and genetics teachers such as Dr. Evelyne Myslinski, Prof. Philippe Carbon, and Prof. Serge Potier. "I also had a lot of structural biology courses, which insisted that the determination of the 3D structure of macromolecular complexes is essential to better understand at the atomic scale cellular processes in health and diseases." During her undergraduate years, Solène had the opportunity to work in Dr. Sylvie Friant's lab, using Saccharomyces cerevisiae as a model to study centronuclear myopathies, which ignited her passion for molecular biology and biochemistry.

Currently, Solène is engaged in a Ph.D. project under the mentorship of Professor Hubert Becker and Dr. Sylvie Friant at IMCBio. Her research focuses on the dynamics and plasticity of the aminoacyl-tRNA synthetases, particularly the S. cerevisiae multi aminoacyl-tRNA synthetase AME complex. This project involves studying the relocalization and non-canonical functions of this complex, which includes the glutamyl- and methionyl-tRNA synthetases linked to the Arc1 cofactor. "By joining this program, I reached my first goal: an exciting PhD project, extra training, funding for abroad internships and meetings, and opportunities to organize summer schools with other Ph.D. students from the IMCBio graduate school."

"The career path to becoming a researcher is highly competitive, but nothing is impossible or unreachable. I don’t know whether I will accomplish my dream of becoming an academic researcher, but I will push my limits to be one of them!"

Beyond research, Solène is deeply committed to science communication and outreach. "I teach enzymology and biochemistry to third-year bachelor's students. As one of my goals is to continue later with teaching activities besides research, it gives me a real insight into this responsibility, and I adore it!" Solène also participates in science festivals, explaining the use of yeast models to study diseases to school students and families. Additionally, as a member of the Women and Science association, she promotes women in scientific and technical careers by visiting high schools and leading workshops.

Solène's favorite RNA is tRNA, closely tied to her research on aminoacyl-tRNA synthetases. She was particularly fascinated by tRNA-derived fragments (tRFs), a new class of small non-coding RNAs that play critical roles in gene expression regulation. Solène highlights an article from Lluís Ribas de Pouplana's lab published in the RNA Journal, which explores how tRFs regulate Adenosine deaminase acting on transfer RNA (ADAT) and its implications for diseases like microcephaly and epilepsy. She also appreciates the paper by Dr. Benoît Masquida from her own lab, which investigates the unique conformations of UNCG family loops in RNA structures.

Solène emphasizes the importance of continuous learning and the privilege of having access to scientific knowledge. "The team and the persons in it make everything. Without them, I would not be sure to be featured today as an RNA society spotlight and I am more than grateful to them." As she continues her journey in RNA research, Solène remains committed to pushing the boundaries of molecular biology and inspiring future generations of scientists.

]]> Actualités du GMGM Focus du GMGM Actualités de l'Équipe Becker Actualités de l'Équipe Friant news-14855 Mon, 23 Oct 2023 16:14:07 +0200 Fête de la Science Lingolsheim 6 au 8 octobre 2023 /en/news/piece-of-news/fete-de-la-science-lingolsheim-6-au-8-octobre-2023 GMGM a organisé et présenté la fête de la Science à Lingolsheim du 6 au 8 octobre 2023. Les membres de l'équipe Friant et Becker ont été impliqués dans divers ateliers :
- environnement: le monde des abeilles et de l'apithérapie
- écologie: la levure pour visualiser l'effet de cocktails de pesticides
- biodiversité: les champignons filamenteux, les observer et les comprendre
- Sciences participatives: Avec le jeu Mendeleieva, découvrez les éléments chimiques de votre vie de tous les jours et des femmes scientifiques étudiant ces éléments.

D'autres ateliers étaient proposés :
- santé: l'effet d'une carence alimentaire (régime) sur la physiologie d'une cellule
- le monde de l'ARN: construisez de l'ARN avec des bases aimantées
- neurosciences: NeuroBioCell, comprendre et observer le fonctionnement des cellules neuronales
- chimie: façonner les molécules pour en faire des micro-machines
- climat: la fresque du climat avec des étudiant-e-s de l'IUT de Schiltigheim
- Physique: l'Année de la Physique avec une exposition de femmes physiciennes
- Comité du Bas-Rhin de La Ligue contre le cancer (Ligue 67): la recherche contre le cancer, L’Agenda scolaire (ma santé, j’en prends soin), comment se protéger du cancer
- Sport dans le cadre des JO2024: Histoire du sport en Alsace

Et des conférences :

Conférence exceptionnelle de Jean-Pierre Sauvage, prix Nobel de chimie 2016

Mercredi 11 octobre à 20h00 : Gymnase Im Sand - Lingolsheim: réservation obligatoire

 

Rencontre-débat avec des sportifs médaillés et un préparateur physique professionnel

Mercredi 4 octobre à 20h00 : Maison des Arts de Lingolsheim "Science et condition physique : les sportifs de haut niveau", avec des sportifs médaillés et un préparateur physique professionnel (rugby, snowboard, tir paralympique) : avec Sylvain Dufour snowboard (Vainqueur de la Coupe du Monde 2014, Médaille d’argent aux Championnats du Monde 2009, 3 participations aux Jeux Olympiques, 4ième au JO d’hiver de 2018); Raphaël Voltz, tir à la carabine (Double vice-champion paralympique aux JO Pékin 2008, médaille d'argent JO Londres 2012, Double champion du monde) ; Romain Ritter, Préparateur physique Strasbourg Alsace Rugby. Animée par le Pr Michel Hasselmann.

 

Comprenez l'apport de la science dans le sport - conférences à la Maison des Arts de Lingolsheim à 20h00 :

  • 3 octobre : "Sport et santé": Cancer et activité physique : Dr Evelyne Lonsdorfer
  • 6 octobre: "Ocytocine : amour, sport et autres addictions sympathiques ...": Pr Marcel Hibert
  • 9 octobre: "Médecine, dopage et sport": Dr John Lenertz
  • 13 octobre: "Sports et Sciences": Créativité, partage et Joie de Vivre: Dr Pierre Antony

 

Rencontre-débat avec des scolaires sur l'histoire du sport en Alsace

Mardi 10 octobre à 14h30 : Avec Denis Jallat > Responsable du Comité de rédaction « histoire » de la revue Sciences sociales et sport. Sébastien Stumpp > Membre du Comité d’histoire des ministères chargés de la Jeunesse et des Sports (CHMJS) Cathy Blanc-Reibel > Ingénieure  CNRS

Pour public scolaire: primaires (CM1/CM2) et collèges - Gymnase Im Sand - Lingolsheim: réservation obligatoire

 

Table-Ronde: Sports, racisme et neuroscience - les comportements racistes dans le sport

Samedi 7 octobre à 14h00 : Gymnase Im Sand – Lingolsheim : avec Odile Rohmer Professeure en psychologie sociale - Jérôme Beauchez Professeur de sociologie et d'anthropologie à l'Université de Strasbourg - Daouada Ba > Président de la commission sport de la LICRA

 

]]> Actualités du GMGM Focus du GMGM Actualités de l'Équipe Becker Actualités de l'Équipe Friant news-13759 Thu, 06 Jul 2023 07:47:41 +0200 Regards sur les ribozymes /en/news/piece-of-news/regards-sur-les-ribozymes Benoït Masquida, directeur de recherche CNRS au laboratoire de Génétique moléculaire, génomique et microbiologie (GMGM) nous explique les multiples facettes des ribozymes, ARN capables d'activité catalytique. Actualités du GMGM Actualités de l'Équipe Becker Focus de l'équipe Becker news-12686 Fri, 04 Nov 2022 14:43:28 +0100 Fête de la Science 2022 à Lingolsheim /en/news/piece-of-news/fete-de-la-science-2022-a-lingolsheim La Fête de la Science s'est déroulée du 7 au 9 octobre 2022 à Lingolsheim https://www.fetedelascience.fr/village-des-sciences-de-lingolsheim Découvrez le monde fascinant de la recherche à travers des ateliers et des conférences:

- environnement: le monde des abeilles et de l'apithérapie

- santé humaine: la levure de boulanger pour mieux comprendre les maladies génétiques rares

- un autre regard sur l'Art: comment on perçoit une œuvre d'art grâce à l'application Ikonikat

- le monde de l'ARN: construisez de l'ARN avec des bases aimantées

- biodiversité: les champignons filamenteux, les observer et les comprendre

- chimie: façonner les molécules pour en faire des micro-machines

- orientation: découvrir les métiers de la recherche scientifique au CNRS

- 2022 Année du Bicentenaire de la naissance de Louis Pasteur: Louis Pasteur microbiologiste

- Comité du Bas-Rhin de La Ligue contre le cancer (Ligue 67): la recherche contre le cancer, L’Agenda scolaire (ma santé, j’en prends soin), comment se protéger du cancer

- Sciences participatives: Avec le jeu Mendeleieva, découvrez les éléments chimiques de votre vie de tous les jours et des femmes scientifiques étudiant ces éléments.

3 équipes du GMGM ont tenu des strands lors de cet évennement.

]]> Actualités du GMGM Actualités de l'Équipe Becker Actualités de l'Équipe Friant Actualités de l'Équipe Vuilleumier news-12102 Tue, 07 Jun 2022 15:28:00 +0200 Junior chairs in the field of research on mitochondria /en/news/piece-of-news/chaires-juniors-dans-le-domaine-de-recherches-sur-les-mitochondries The Laboratory of Excellence (LabEx) MitoCross announces the call for applications for two junior chairs in the field of research on mitochondria. See details HERE.

]]> Actualités du GMGM Actualités de l'Équipe Becker Actualités de l'Équipe Tarassov-Smirnov Actualités de l'Équipe Schacherer news-10347 Thu, 15 Apr 2021 16:31:00 +0200 Publication Equipe Friant et Becker : CEX1 /en/news/piece-of-news/publication-equipe-friant-et-becker-cex1 New publication : Cex1 is a component of the COPI intracellular trafficking machinery Ludovic Enkler, Bruno Rinaldi, Johan Owen de Craene, Philippe Hammann, Osamu Nureki, Bruno Senger, Sylvie Friant, Hubert D Becker

 

COPI (coatomer complex I) coated vesicles are involved in Golgi-to-ER and intra-Golgi trafficking pathways, and mediate retrieval of ER resident proteins. Functions and components of the COPI-mediated trafficking pathways, beyond the canonical set of Sec/Arf proteins, are constantly increasing in number and complexity. In mammalian cells, GORAB, SCYL1 and SCYL3 proteins regulate Golgi morphology and protein glycosylation in concert with the COPI machinery. Here, we show that Cex1, homologous to the mammalian SCYL proteins, is a component of the yeast COPI machinery, by interacting with Sec27, Sec28 and Sec33 (Ret1/Cop1) proteins of the COPI coat. Cex1 was initially reported to mediate channeling of aminoacylated tRNA outside of the nucleus. Our data show that Cex1 localizes at membrane compartments, on structures positive for the Sec33 α-COP subunit. Moreover, the Wbp1 protein required for N-glycosylation and interacting via its di-lysine motif with the Sec27 β'-COP subunit is mis-targeted in cex1Δ deletion mutant cells. Our data point to the possibility of developing Cex1 yeast-based models to study neurodegenerative disorders linked to pathogenic mutations of its human homologue SCYL1.

Biology Open 2021 10: bio058528 doi: 10.1242/bio.058528 Published 22 March 2021

]]> Actualités du GMGM Publications du GMGM Actualités de l'Équipe Becker Actualités de l'Équipe Friant news-9592 Thu, 10 Sep 2020 14:11:00 +0200 Cover of the Elife journal /en/news/piece-of-news/groupe-becker-actualites-du-cnrs New publication from DyPS team, in cover of Elife.

A fragmented bi-genomic GFP to study multi-localised proteins

Our team and its colaborators created a yeast strain expressing GFP (Green Fluorescent Protein) fragmented bi-genomic (encoded by both nuclear and mitochondrial genome) enabling fluorescence to happen only for a mitochondrial subpopulation of proteins  that are localised in both cytoplasm and mitochondria. These results were published in Elife.

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